Fluorescence-aided molecule sorting: analysis of structure and interactions by alternating-laser excitation of single molecules

Proceeding of the National Academies of Sciences USA 101 (2004) 8936-8941

Authors:

A Kapanidis, Lee NK, Laurence TA, Doose S

Photon Arrival-Time Interval Distribution (PAID): A Novel Tool for Analyzing Molecular Interactions

The Journal of Physical Chemistry B American Chemical Society (ACS) 108:9 (2004) 3051-3067

Authors:

Ted A Laurence, Achillefs N Kapanidis, Xiangxu Kong, Daniel S Chemla, Shimon Weiss

Single-molecule analysis of sigma factor release

BIOPHYS J 84:2 (2003) 7A-7A

Authors:

A Kapanidis, E Margeat, T Laurence, NK Lee, S Doose, J Mukhopadhyay, E Kortkhonjia, R Ebright, S Weiss

Fluorescence resonance energy transfer (FRET) in analysis of transcription-complex structure and function.

Methods Enzymol 371 (2003) 144-159

Authors:

Jayanta Mukhopadhyay, Vladimir Mekler, Ekaterine Kortkhonjia, Achillefs N Kapanidis, Yon W Ebright, Richard H Ebright

The power and prospects of fluorescence microscopies and spectroscopies.

Annu Rev Biophys Biomol Struct 32 (2003) 161-182

Authors:

Xavier Michalet, Achillefs N Kapanidis, Ted Laurence, Fabien Pinaud, Soeren Doose, Malte Pflughoefft, Shimon Weiss

Abstract:

Recent years have witnessed a renaissance of fluorescence microscopy techniques and applications, from live-animal multiphoton confocal microscopy to single-molecule fluorescence spectroscopy and imaging in living cells. These achievements have been made possible not so much because of improvements in microscope design, but rather because of development of new detectors, accessible continuous wave and pulsed laser sources, sophisticated multiparameter analysis on one hand, and the development of new probes and labeling chemistries on the other. This review tracks the lineage of ideas and the evolution of thinking that have led to the actual developments, and presents a comprehensive overview of the field, with emphasis put on our laboratory's interest in single-molecule microscopy and spectroscopy.